Investigators at Cornell University (Ithaca, NY, USA) delineate that Cas3 from Thermobifida fusca was found to be guided specifically towards Cascade-bound target DNA in the presence of an optimal Protospacer Adjacent Motif (PAM) sequence, and through physical interplay with the CasA component of the Cascade and the non-complementary strand of the ds-DNA substrate. This means that after using the CRISPR mechanism to identify the target DNA, the Cas 3 nuclease erased DNA continuously, for up to 100 kilobases with 13%–60% editing efficiency.
Dr. Ailong Ke, professor of molecular biology and genetics at Cornell University. Said “Our tools can be made to target these viruses very specifically and then erase them very efficiently. In theory, it could provide a cure for these viral diseases.”