PB transposon mutagenesis identifies malaria genes and drug targets

A team of genome researchers took dominance of the curious nucleotide composition of Plasmodium falciparum DNA to produce mutant strains of the malaria parasite. Pinpoint essential genes and potential drug targets. The extreme adenine-thymine (AT)-bias of the P. falciparum genome has hampered genetic studies through earmark looms such as homologous recombination or CRISPR/Cas9, and only a few hundred P. falciparum mutants have been empirically generated in the past decades.Using piggyBac mutagenesis, our new genetic tool, we have functionally characterized nearly all of the parasite’s genes.
The enriched A-T composition of the P. falciparum genome presented numerous piggyBac transposon insertion targets within both gene coding and noncoding flanking sequences.Investigators at the University of South Florida (Tampa, USA) exploited the AT-richness of the P. falciparum genome by using piggyBac transposon insertion sites to achieve saturation-level mutagenesis.
Original Link: https://www.biotechdaily.com/genomics-proteomics/articles/294773500/pb-transposon-mutagenesis-identifies-malaria-genes-and-drug-targets.html